Scientists at NCCS have developed an in-vitro anti-diabetic drug screening system based on glucose transporter 4 (GLUT4) activity in a novel cell line (CHO-HIRc-mycGLUT4eGFP). Using this system, GLUT4 translocation under stimulation can be visualized by live cell imaging and captured real-time for qualitative and quantitative analysis of GLUT4 on the cell membrane.
This real time, visual, cell-based, qualitative and quantitative system can be used for screening large number of natural products, synthetic compounds or derived products for Glut4 translocation modulation (or anti-diabetic) activity.
Technology Readiness: TRL B1 (In-vitro efficacy/ results demonstrated)
Technology Status: Proprietary know-how
Technology Availability: Know-how available for transfer/ co-development with partners.
References:
- Real Time qualitative and quantitative GLUT4 translocation assay, Methods in Enzymology: Volume:505, 2012 (Article).
- Demonstration of a visual cell-based assay for screening GLUT4 translocation modulators in real time, Journal of Biosciences, 2010, 35: 525-31 (Article).
- The hypoglycaemic activity of fenugreek seed extract is mediated through the stimulation of an insulin signaling pathway, British Journal of Pharmacology, 2005, 146:41-45 (Article).