Category Archives: Stem Cells

Endocytosis in deciding fate of Embryonic Stem Cells

Scientists at NCCS are investigating the role of endocytosis and vesicular transport in deciding the future state of an undifferentiated embryonic stem cell. Using experimental techniques like large scale siRNA screening, reprogramming assays and biophysical manipulation of mouse embryos, they are studying how modifications to cellular or vesicular transport determines whether an embryonic stem cell maintains pluripotency or differentiates into a specific cell type. Continue reading

Tapping into Pluripotent Differentiation Potentials of Embryonic Stem Cells

Using the pluripotent embryonic stem cells as a model system, scientists at NCCS are investigating the epigenetic factors and molecular determinants which are involved in deciding and controlling differentiation of stem cells into specific cellular lineages. Continue reading

An Elegant Model for Studying Early Embryonic Development

Scientists at NCCS are studying the early embryonic development using embryonic stem cells derived from the blastula stage embryo. These embryonic stem cells possess unique characteristics of indefinite self renewal and pluripotency; and are capable of precisely recapitulating the early embryogenic development, thereby serving as an elegant model for studying the developmental events occurring during embryogenesis. Continue reading

Self-propagating Glioblastoma Model in a Petri-dish

Scientists at NCCS have developed a novel tumor cell co-culture protocol and system for understanding the role of glioma stem cells (GSC) in mediating tumor functions in Glioblastoma (GBM). GBM is a highly invasive malignant tumor with high incidence of therapy resistance and tumor relapse after surgery. This relapse is due to repopulation of tumor by GSCs and is characterized by existence of GSCs in close proximity with endothelial cells (EC). In order to study the GSC-EC interactions, this innovative self-propagating GSC-EC co-culture system was developed as a highly replicating in-vitro model of GBM. Continue reading

Dendritic Cells for Cancer Immunotherapy

Scientists at NCCS have developed a novel two-step method for large-scale generation of mature, functional Dendritic Cells (DC) from Umbilical cord blood (UCB). This technology will aid in development of dendritic cell based vaccines for cancer immunotherapy. Continue reading

Indigenous Protocol for Cryopreservation of Cord Blood

Umbilical cord blood (UCB) collected from umbilical cord and placenta following child birth, is a rich source of stem cells which are preserved and harvested for treatment of many diseases and for use in stem cell research. Efficient preservation of cord blood cells is crucial for availability of viable stem cells at the time of need. Scientists at NCCS have developed a unique technique for cryopreservation of UCB cells for banking and research. Continue reading

Cell Bandages for Tissue Repair in Diabetic Wounds

Wound healing in diabetic conditions is severely impaired and is a major cause of extremity amputation. A cellular, tissue engineered, wound-healing product comprising of a novel matrix of electro-spun Polycaprolactone-Gelatin nano-fiber scaffold (PCG) with embedded Bone Marrow or Peripheral Blood-derived endothelial progenitor cells (BMPC) system has been developed by scientists at NCCS, for accelerated and fibrosis-free wound healing in diabetic conditions. Continue reading

Ready-to-use Cell-free Secretomes for Wound Healing

Secretomes are protein molecules that are secreted or released from cells and are actively involved in control and regulation of biological processes in the body like wound healing, skin formation etc. Scientists at NCCS have created a novel skin repair product using these secretomes. Continue reading

Platform for Identifying Heterogeneity of Cancer Cells

Cancer cells remaining undetected in the body following treatment regimens can cause recurrence. Recent studies suggest that this debilitating issue could be a result of heterogeneous characteristics of cancer cells; thus residual cells are likely to be drug resistant and/or not targeted by the initial treatment. Scientists at NCCS believe that identifying the heterogeneity of the cancer cells and providing a targeted therapy specific for different cell fractions can resolve this issue. Continue reading

Novel Cancer Drug Screening Platform

Scientists at NCCS have developed a novel platform for screening of potential anti-tumor agents, compounds and extracts, based on 3-dimensional Multi-Cellular Spheroids derived from tumor cells and prostate apoptosis response (PAR)-4 indicator that is linked to cell death. Continue reading

In-vitro Bone Marrow Niche Platform and Pre-clinical Screening Kit

An artificial bone marrow microenvironment (ABME) platform for enhancing and regulating in-vitro as well as in vivo formation of stem cells has been created by scientist at NCCS, Pune. This technology can be incorporated into high throughput and micro-fluidic devices for drug screening and in-vitro toxicology testing. Continue reading

An Overview of NCCS Capabilities

Capability Area NCCS Scientists/ Consultants
Cancer Biology Dr. Sharmila Bapat, Dr. Padma Shastry, Dr. Anjali Shiras, Dr. Mohan Wani, Dr. Gopal Kundu, Dr. Samit Chattopadhyay, Dr. Manoj Kumar Bhat, Dr. Sandhya Sitaswad, Dr. Girdhari Lal, Dr. Srikanth Rapole, Dr. Manas Santra
Cell Biology Dr. Shekhar Mande, Dr. Mohan Wani, Dr. Debashis Mitra, Dr. Prakash Deshpande, Dr. Jomon Joseph,  Dr. Bhaskar Saha, Dr. Milind Patole, Dr. Arvind Sahu, Dr. M. V. Krishnasastry, Dr. Vasudevan Seshadri, Dr. Radha Chauhan, Dr. Janesh Kumar, Dr. Girdhari Lal, Dr. Shailza Singh, Dr. Deepa Subramanyam
Genetics Dr. Sharmila Bapat, Dr. Anjali Shiras, Dr. Samit Chattopadhyay, Dr. Jomon Joseph, Dr. Vasudevan Seshadri, Dr. Manas Santra, Dr. Vidisha Tripathi
Immunology  Dr. Mohan Wani, Dr. Bhaskar Saha, Dr. Arvind Sahu, Dr. Girdhari Lal, Dr. Shailza Singh
Metabolic Diseases Dr. Manoj Kumar Bhat, Dr. Sandhya Sitaswad
Microbial Biology Dr. Milind Patole, Dr. Yogesh Shouche, Dr. Shailza Singh
Neuroscience Dr. Amitabha Majumdar, Dr. Janesh Kumar
Stem Cells Dr. Vaijayanti Kale, Dr. Lalita Limaye, Dr. Anjali Shiras, Dr. Nibedita Lenka, Dr. Deepa Subramanyam